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首頁> 外文OA文獻(xiàn) >Characterization of transcription organization and analysis of unique expression patterns of an alkyl hydroperoxide reductase C gene (ahpC) and the peroxide regulator operon ahpF-oxyR-orfX from Xanthomonas campestris pv. phaseoli.
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Characterization of transcription organization and analysis of unique expression patterns of an alkyl hydroperoxide reductase C gene (ahpC) and the peroxide regulator operon ahpF-oxyR-orfX from Xanthomonas campestris pv. phaseoli.

機(jī)譯：Xanthomonas campestris pv的烷基氫過氧化物還原酶C基因（ahpC）和過氧化物調(diào)節(jié)子操縱子ahpF-oxyR-orfX的轉(zhuǎn)錄組織特征和獨(dú)特表達(dá)模式的分析。菜豆。

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摘要

We have analyzed the transcription organization of ahpC, ahpF, oxyR, and orfX from Xanthomonas campestris pv. phaseoli. ahpC was transcribed as a monocistronic 0.6-kb mRNA, while ahpF-oxyR-orfX were transcribed as a polycistronic approximately 3.0-kb-long mRNA. The novel transcription organization of these genes has not observed in other bacteria. Western analysis showed that oxidants (peroxides and superoxide anions), a thiol reagent (N-ethylmaleimide), and CdCl2 caused large increases in the steady-state level of AhpC. Growth at alkaline pH also moderately induced AhpC accumulation. Thermal and osmotic stresses did not alter the levels of AhpC. Northern blotting results confirmed that oxidant- and CdCl2-induced AhpC accumulation was due to increased levels of ahpC transcripts. Analysis of oxyR expression revealed a unique pattern. Unlike other bacterial systems, peroxides and a superoxide generator induced accumulation of OxyR. Northern blotting results confirmed that these oxidants induced expression of oxyR operon. This novel regulatory pattern could be generally important. The transcription organization and patterns of chemicals and stress induction of ahpC and oxyR differed from those of other bacteria and are likely to be important for X. campestris pv. phaseoli survival during exposure to oxidants.
機(jī)譯：我們已經(jīng)分析了來自油菜黃單胞菌PV的ahpC，ahpF，oxyR和orfX的轉(zhuǎn)錄組織。菜豆。 ahpC被轉(zhuǎn)錄為單順反子0.6-kb mRNA，而ahpF-oxyR-orfX被轉(zhuǎn)錄為約3.0-kb長的多順反子mRNA。這些基因的新型轉(zhuǎn)錄組織尚未在其他細(xì)菌中觀察到。 Western分析表明，氧化劑（過氧化物和超氧陰離子），硫醇試劑（N-乙基馬來酰亞胺）和CdCl2引起AhpC穩(wěn)態(tài)水平的大幅提高。在堿性pH值下生長也適度誘導(dǎo)了AhpC的積累。熱應(yīng)力和滲透脅迫不會(huì)改變AhpC的水平。 Northern印跡結(jié)果證實(shí)了氧化劑和CdCl2誘導(dǎo)的AhpC積累是由于增加了ahpC轉(zhuǎn)錄物的水平。 oxyR表達(dá)的分析揭示了一個(gè)獨(dú)特的模式。與其他細(xì)菌系統(tǒng)不同，過氧化物和超氧化物生成物會(huì)誘導(dǎo)OxyR積累。 Northern印跡結(jié)果證實(shí)了這些氧化劑誘導(dǎo)了oxyR操縱子的表達(dá)。這種新穎的監(jiān)管模式通?？赡芎苤匾?。 ahpC和oxyR的化學(xué)物質(zhì)的轉(zhuǎn)錄組織和模式以及應(yīng)激誘導(dǎo)與其他細(xì)菌的轉(zhuǎn)錄組織和模式不同，并且可能對(duì)campestris pv至關(guān)重要。菜豆在暴露于氧化劑期間的存活率。

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